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Another reporter of active Wnt/β-catenin signaling is Axin2 expression, which acts as a negative feedback inhibitor of the pathway (Lustig et al., Axin2 is highly expressed in the periotic mesenchymal cells that surround the cochlear duct in a spatiotemporal pattern remarkably different from that of Lgr5 and TCF/LEF reporters (Chai et al., β -catenin in Tbx1 -expressing periotic mesenchymal cells led to loss of Axin2 expression, reduction in the overall cell number and subsequent differentiation into fibrocytes (Bohnenpoll et al., Tbx1 -expressing cells induced a mitogenic response
