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Importantly, the high consumption of methionine outcompetes regeneration and renders these cancer cells auxotrophic to methionine and vulnerable to pharmacological inhibition [240] (Figure 6)
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The proteins on filters were digested for 2 h at room temperature using 0.5 µg Lys-C (Wako Chemicals) and for 16 h at 37 °C with 1 µg trypsin (Promega)
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