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Cells were then washed and resuspended in 200 cL flow cytometry staining buffer and analyzed using FACSAria III cell sorter (BD Biosciences, USA)

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Using this technique, we found a significant increase in NO levels and a reprogramming of the SNO-proteome in the brain of Shank3 InsG3680 (+/+) mouse model of ASD [171]

The assay will exclusively recognize both natural and recombinant Hu Thyroid Peroxidase