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[DOI] [PMC free article] [PubMed] [Google Scholar] 104.Sio T.T., Blanchard M.J., Novotny P.J., Patel S.H., Rwigema J.C.M., Pederson L.D., McGee L.A., Gamez M.E., Seeger G.R., Manterson J.A., et al

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Cells were immediately recovered in 30 ml of prewarmed 2× YT supplemented with 25 mM glucose, and replicate reactions were pooled at this stage