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The levels of VEGF-A protein in serum-free culture supernatants (50 µg/ml) from EBC-1 cells treated with or without siRNAs were measured using the Human VEGF-A ELISA Kit (RayBiotech, Inc.) according to the manufacturer's instructions as described previously (8)
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Peptides tend to work via an indirect path such as upregulating endogenous antioxidant pathways or enhancing mitochondrial repair
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