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Have been putting it down to ghastly British winter -more ghastly than usual, and not much sign of spring yet
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a combination of 1mM IBMX, 1 µM dexamethasone, and 10 µg/ml of insulin in media were added to fully confluent 3T3-L1 fibroblasts for three days (days 0–3) to initiate differentiation
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