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These findings justify the recent conclusions by the American Gastrointestinal Association (6) and others (7, 8) (quoted in the Introduction) that in patients taking acid blockers or metformin, routine screening or monitoring of vitamin B-12 status and taking of vitamin B-12 supplements above the RDA are not warranted

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The following day, overnight cultures were diluted 1:100 into fresh 2×YT medium (250–1,000 ml) containing kanamycin and incubated at 37 °C until reaching mid-log phase (optical density at 600 nm (OD 600 ) ≈ 0.4–0.7)