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RP-HPLC purification was conducted by collection under the same conditions on a semi-prep scale using a Grace Vydac Denali C18, 120 Å, 150 × 10.00 mm, and 5 μm column at a 5 mL/min flow rate
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GHK-Cu has been studied extensively in preclinical research examining dermal fibroblast activity, collagen and glycosaminoglycan synthesis, matrix metalloproteinase modulation, and angiogenesis
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