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After fixation of the biopsy specimens, free floating 50-ΞΌm-thick sections were cut and stained by using rabbit anti-protein gene product 9.5 antibody (Dako Netherlands BV, Eindhoven, the Netherlands) and visualized using a goat anti-rabbit Alexa fluor 488 antibody (Invitrogen,/Life Technologies, Carlsbad, CA, USA)
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