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Ninety-six-well plates with the fixed cells were examined using ImageXpress (Molecular Devices)

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Among the diverse cell death modalities implicated in stroke pathogenesis, ferroptosis-an iron-dependent programmed cell death pathway characterized by lipid peroxidation-has emerged as a critical driver of ischemic neurodegeneration ( First described in 2012, ferroptosis is characterized by mitochondrial cristae shrinkage, glutathione peroxidase 4 (GPX4) inactivation, and lethal lipid peroxide accumulation through iron-dependent Fenton reactions (Li, 2020)