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The mitochondrial pellet was resuspended in 90 μL of 1X TD (49.99 mM Tris base [Research Products International, T60040-5000], 274.13 mM NaCl, 20.12 mM KCl, 13.95 mM Na 2 HPO 4 [Sigma-Aldrich, S5136]) and split between three 1.5 microcentrifuge tubes