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Mechanical rupture (using different procedures such as bead-milling, ultrasonication, or high-pressure homogenization) is the conventional procedure applied to provoke the release of biomolecules located in the cell cytoplasm ( Several studies have demonstrated the benefit of the application of a PEF treatment for the subsequent extraction of different compounds from yeast, such as proteins, carotenoids, glutathione, and recombinant proteins with antitumoral effect ( Release of mannoproteins into the medium in which the yeast biomass was re-suspended required enzymatic hydrolysis of the cell wall
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