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In fact, the analysis of the CD spectra, made as described under Materials and Methods section, showed an increased value of β-sheet (39%) in the reduced protein compared to the 25% of the native form while the α-helix slightly decreases from 12% to 10% in the reduced form

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The selection of the enzymatic hydrolysis conditions was based on preliminary experimental results ( 2.3 Animals and experimental design All 12-month-old male Wistar rats were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (Nanjing, China)

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