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coli XL1-blue using heat shock transformation followed by mini prep using peqGOLD plasmid MiniPrep kit (VWR International) for the confirmation of the transformation using restriction enzyme digestion ( Sma I and Hin dIII) of the plasmid followed by agarose gel electrophoresis
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Size:10 ml
Histological analysis of adipose tissue from 5-Amino-1MQ-treated animals reveals increased presence of multilocular brown-like adipocytes, increased mitochondrial density, elevated expression of UCP1 and other thermogenic markers, and reduced adipocyte size
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