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A healthcare provider will decide this

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For cell fractionation before IP, cells were gently lysed (in 20 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl 2 , 0.3% (v/v) NP-40, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1 mM EGTA, with freshly added 1 mM sodium vanadate, 50 mM sodium fluoride and protease-inhibitor cocktail)