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2.13 Yeast two-hybrid complementation assay Recombinant plasmids, including pGADT7 and pGBKT7, were co-transformed into yeast ( 2.14 Interaction protein binding-site analysis AlphaFold 2 software was used to model three-dimensional structures of interaction protein ( ( 2.15 Statistical analysis Three separate experiments or biological replicates were analysed using IBM SPSS 25.0 (IBM Corp., NY, USA), using a one-way ANOVA followed by Duncan’s multiple comparison test

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