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Measurements with the UHP-HILIC-MS method employed 1 mM dithiothreitol that can disrupt the disulfide bonds in NNMT protein and change the three-dimensional structure, while the SAHH-coupled fluorescent assay employed a complex reaction buffer containing SAHH, adenosine deaminase, ThioGlo, MgCl 2 , potassium phosphate, EDTA that potentially interfere the reaction kinetics [17]