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Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set zero with distilled Preheat Reagent II in 37℃ (mammal cell) or 25℃ (other species) water bath for 30 Blank tube determination: take micro glass cuvette, add 20 μL of distilled water, 140 μL of Reagent II, 40 μL of Reagent III in turn, mix well, place for 2 minutes, and measure 412 nm absorbance AB
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