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The supernatant was discarded and the mitochondrial pellet was resuspended in 1 ml ice-cold IB cells -2 and centrifuged at 10,000 g for 10 min at 4 °C

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Disruption of ROS homeostasis either pharmacologically or genetically has been shown to cause abnormal tubulin polymerization or impaired cell plate formation in Arabidopsis and wheat root tip cells, resulting in defective cytokinesis (Livanos et al., 2012)