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After 1 h incubation in the dark at room temperature, 500 µl 1% (v/v) 1-fluoro-2,4-dinitrobenzene was added and the mixture was incubated in the dark for 1 h at room temperature and then overnight at 4 °C

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These positive feedback loops reinforce the notion that early intervention is the optimal strategy for potentially reversing or dramatically slowing T2D progression