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PLGA is a hydrophobic polymer, which is more suited to encapsulate hydrophobic rather than hydrophilic agents via two general methods 4 : (1) Nanoprecipitation, in which PLGA and agents to be encapsulated are dissolved in a “good solvent” and then co-precipitate into nanoparticles upon addition into a non-solvent for both

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As an alternative to HPLC, ion exchange chromatography also could be performed