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However, an analysis of data from the Vitamin D and Omega-3 Trial (VITAL) found, among 1,032 participants with migraines, those who received 2,000 IU (50 mcg) vitamin D3 per day reported no changes in migraine frequency or severity compared with placebo during almost five years of treatment (Rist 2021)

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A 2016 Nature Scientific Reports study showed GHK-Cu produced no cytotoxicity to human skin cells at concentrations up to 5,800 micromolar over 72 hours and did not induce inflammatory biomarkers unlike other copper delivery methods