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Catalytic Residues Roles Chemical Components proton transfer, ingold: bimolecular nucleophilic substitution, enzyme-substrate complex formation, intermediate formation, overall product formed, cofactor used Step 6

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However, due to the high binding affinity of biotin to avidin, eluting your biotinylated protein from an immobilized streptavidin can be difficult

optimized to yield up to 10mg of soluble GST tagged protein