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However, the mass of peak 3 reacted in D 2 O (1751.8 Da, observed ion: m / z 876.9, z = 2) was 1 Da heavier than that reacted in H 2 O (1750.8 Da, observed ion: m / z 876.4, z = 2), indicating deuterium incorporation during the labeling reaction in D 2 O and therefore the presence of an isoAsp residue
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