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injections of 100 nmol kg −1 MK-801 ( n = 8 mice), 100 nmol kg −1 GLP-1 ( n = 8 mice), 100 nmol kg −1 GLP-1–MK-801 ( n = 8 mice) or vehicle (isotonic saline, n = 8 mice) at a volume of 5 µl g −1

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▼ Standard washout periods range from 4–6 weeks after an 8–12 week cycle to allow AMPK receptor sensitivity and NNMT enzyme activity to return to baseline