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In vivo protein interaction analysis Cell extracts from asynchronously growing cultures were prepared in EBX buffer (50 mM HEPES-KOH pH 7.5, 100 mM KCl, 2.5 mM MgCl 2 , 10% glycerol, 0.25% Triton X-100, 0.5 mM TCEP, protease inhibitors, RNase and benzonase) using glass beads breakage in a cooled Multi-Beads Shocker (Yasui Kikai)

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[DOI] [PubMed] [Google Scholar] 151.Kumar P, Kumar D, Sikka P, Singh P